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International Journal of Clinical & Medical Microbiology Volume 1 (2016), Article ID 1:IJCMM-104, 4 pages
Research Article
The most Common Causes of Cervico-vaginal Infections Among Sudanese Women Complainingof Vaginal Discharge

Osman Imam Ahmed1, Ihab Hamed Nourein2, Moawia A Elbadri3, Ahmed Elbasheer Abdelgadir4, Ietimad AM Ayed5 and Mohammed HF Shalayel6*

1Department of Histopathology, College of Medical Lab, University of Medical Sciences, Sudan
2Department of Histopathology, College of Applied Medical Sciences, Najran University, KSA
3Department of Microbiology, College of Medicine, Najran University, KSA
4Department of Hematology, College of Medical Laboratories, University of Medical Sciences, Sudan
5Pharmacology Department, Faculty of Medicine and Health Sciences, Omdurman Islamic University, Sudan
6Professor of Biochemistry and Specialist of Dermatology and Venereology, College of Medicine, Najran University, KSA
Mohammed HF Shalayel, Professor of Biochemistry and Specialist of Dermatology and Venereology, College of Medicine, Najran University, KSA; E-mail:
07 December 2015; 05 March 2016; 07 March 2016
Ahmed OI, Nourein IH, Elbadri MA, Abdelgadir AE, Ayed IA, et al. (2016) The most Common Causes of Cervico-vaginal Infections Among Sudanese Women Complainingof Vaginal Discharge. Int J Clin Med Microbiol 1: 104. doi:


Background: Cervico-vaginal infection is one of the most common problems in clinical medicine. This study aimed to the most common causes of cervical-vaginal infections among Sudanese women complaining of vaginal discharge.
Method: Cross-sectional hospital based study that had carried out on one hundred non-pregnant women complaining of vaginal discharge attended at gynecology department – Khartoum teaching hospital. All subjects had signed informed consent. Smear from all collected samples were prepared and stained with Papanicolaou Stain for cytological evaluation. Descriptive and analytical statistics (chi – square test) were applied using SPSS (Version 20).
Results: The result showed that the majority of subjects were from central and northern of Sudan. The mean age of women was (29) The most common microorganisms seen were bacilli bacteria (49%), followed by candida (18%), Actinomycesisraelii, Leptothrix spp. and Gardnerellavaginalis were (2%), (2%), and (1%) respectively. Statistically no significant association between infection and age group, circumcision, geographical location, and occupation, p value of all these variables was above than significant level 0.05.
Conclusion: The most prevalent infections were bacterial vaginosis and candida albicans. This is consistent with the results of many studies although other studies reported different results.

1. Introduction

Cervico-vaginal infections are one of the most common problems in clinical medicine. The diagnosis of vaginal discharge is based on a History, Physical examination, and a few simple diagnostic tests [1]. Some patients with cervicitis note a purulent vaginal discharge, deep dyspareunia, and spotting after intercourse, while others may be symptom-free. The most common infections are Gardnerella vaginalis Candidia albicans and Trichomona vaginalis that are responsible for 90% of the cases of diseases infectious origin [2,3].Candida causes intense itching with a cheesy, dry discharge. Gardnerella causes a foul-smelling, thin white discharge. Trichomonas gives irritation and frothy white discharge. Chlamydia may cause a purulent vaginal discharge, post-coital spotting, and deep dyspareunia. Gonorrhea may cause a purulent vaginal discharge and deep dyspareunia [4].

Papanicolaou stain (Pap-smear or Pap test) is a cytological method used for screening of cervical cancer and some infectious disease like Gardnerellavaginalis, Candidia albicans and Trichomonasvaginalis by identification either of the organism or of characteristic cytological cellular changes [5,6]. The Pap test is easy to perform, and relatively inexpensive, as well as large number of women can examined by Pap –smear annually [8]. Thus, this technique can become an important tool and the easiest method for detection of cervico-vaginal infections, especially, in developing countries [4,9]. This study aimed to show the causes of cervico-vaginal infections among Sudanese women with vaginal discharge concerning their age, place of residence, and circumcision using Pap smear. The purpose of this study is to assess the clinical efficacy of tigecycline in hospitalized patients.

2. Methodology

Study design: In this cross sectional hospital based study, one hundred cervico-vaginal samples collected from Sudanese women complain of vaginal discharge attended at gynecology department – Khartoum teaching hospital from different places of Sudan. All patients signed an informed consent form.

Sample collection: Cervico-vaginal specimens were collected by expert gynecologist using spatula, the collected samples were spread on clean microscopic slides.

Papanicolaou stain: The smears were fixed immediately in 95% ethanol for 15 minutes, and then rehydrated through descending grades of alcohols 80%, 70%, 50%, and washed with distilled water. Then they were stained with papanicolaou stain using slandered Pap stain procedure.

Data Analysis: Statistical Package for Social Sciences (SPSS) version 20 was used in this study, to analyze, descriptive statistics such as frequency tables, mean. Chi-square test was applied to find the association between two variables. The significance level of 0.05 was considered for statistical analysis.

3. Results

The age of patients ranged from 17 to 50 years (median 29 years) with a mean of 31.5 ± 16.15 years. Most of cases included in this study were in the 3rd decade of life, this group represents 41%of total study population. Out of 100 women, 95women were circumcised and the remaining five women were non-circumcised. Concerning the geographical location, 47women were from Northern, 41from Central, 8from Eastern, 2 from Western and 2 from Southern Sudan as described in Table 1.The most common type of infection was bacterial vaginosis (54%) followed by candida infection which represented18% of the studied cases while the remaining 28%had non-infected vaginal discharge (figure 1).

table 1
Table 1: Distribution of cases of cervico-vaginal infections based on demographic characteristics.
figure 1
Figure 1: Distribution of vaginal infection among study population.

The cytological features of the types of infection were illustrated in Figure 2.

figure 2
Figure 2: Pap smear showing Smear showing; A- Bacterial Vaginosis; BActinomycesisraelii; C- Candida Albicans; D-Leptothrix sp.

Twenty six % of circumcised women were negative (non-infected), 48% had bacilli infection, 1% had Actinomyces, 2% had leptothrix, 1% had Gardinellavaginalis and 17% had Candida albicans. Two % non-circumcised were negative (non-infected), 1% had bacilli infection, 1% had actinomyces, and 1% had Candida albicans as shown in table 1.

In this study, the major cause of cervico-vaginal infection was bacterial vaginosis(bacilli) followed by candidiasis seen in women came from Central and Northern Sudan (table 1).

Regarding the occupation of women, the majority of them were housewives.This group represents 73% of the total cases. 24% of them were negative, 28% had bacilli infection, 2% had actinomyces, 2% had leptothrix, 1% had Gardenellavaginalis and 16% had vaginal candidiasis. The employees represented 27% of the participants, 4% were negative, 21% had bacilli infection and 2% had candida infection. 51% of the studied cases live in Khartoum city of whom 13% were negative, 25% had bacilli infection, 1% had leptothrix, 1% had Gardenellavaginalis and 11% had vaginal candidiasis. 27% of cases came from Omdurman city of whom 6% were negative, 12% had bacilli infection, 1% had leptothrix and 3% had candidiasis. 27% of participants live in Khartoum north (Bahri) of whom 9% were negative, 12% had bacilli infection, 1% had actinomyces, 1% had leptothrix and 4% had vaginal candidiasis. Concerning the educational level, 79% of the study participants were at the secondary level of education. This group represents the majority of cases, while 11% of them were graduated. 4% of the women were illiterate and 6% of them stopped their education at the primary level.

4. Discussion

Vaginal discharge is one of most frequent gynecological problems encountered in females especially during their reproductive stage [10]. The microbiological flora of the lower female genital tract provides a dynamic, complex example of microbial colonization, the regulation of which is not fully understood. When an exogenous bacterial species, with its array of virulence factors, is introduced into the host, disease does not always occur. Conversely, under selected conditions, commensal endogenous bacteria—for example, Gardnerella vaginalis and group B streptococci can participate in disease processes [11].

This study has shown that most cervico-vaginal infections were attributable to bacterial vaginosis (54%) and vaginal candidiasis (18%).These findings to some extentis in agreement witha previous study in which the prevalence of bacterial vaginosis and vaginal candidiasis among women with vaginal discharge was (40%) and (25%) respectively [12].

Our findings differed from the results of a study carried by Maria et al 2014in Iran who reported that most cervical-vaginal infections were attributable to Candida and then Trichomonas and Gardnerella [4]. Other studies reported that the prevalence of bacterial vaginosis and vaginal candidiasis varies from 15- 61% and 14 – 30% respectively [12-15]. These differences are due to different study populations, various climate, socio-economic and cultural conditions, different sensitivity of diagnostic tests, applying different diagnostic methods [4,13,14,15].

Our findings revealed that women aged up to 30 years had the highest prevalence rates of cervical-vaginal infection by bacilli bacteria, Actinomyces and Leptothrix while the highest rates of prevalence of cervico-vaginal infection by candida was observed in the women aged 41 to 50 years. This is consistent with the result of many previous studies [3,4,13,16].

Akingbade et al. reported that the prevalence of Candida albicans amongst women attending antenatal and gynecology health centers in Abeokuta, Nigeria was 24.4% [10].

Bacterial vaginosis is best described as a disruption of the vaginal microbiota or as a vaginal bacterial dysbiosis. It is characterised by a reduction or a replacement of the protective Lactobacillus species by an overgrowth of other anaerobic bacteria [17].

Our study showed that there was no significant association between cervico-vaginal infections with age group, circumcision, and occupation (p- value of these variables were 0.481, 0.137 and 0.791 respectively). This findings support the result of the study carried by Rathod et al., who did not find strong evidence for associations between sociodemographic characteristics and the diagnosis of vulvovaginal candidiasis [18].

Xang et al. (2009) reported that the three most frequent reproductive tract infections (RTIs) were endocervicitis, bacterial vaginosis (BV) and trichomoniasis, with prevalence’s of 41.7%, 12.0% and 4.5%, respectively. Multiple infections were prevalent among our sample. 20.4% (10,854) of our sample had two RTIs and 8.8% (4713) had at least three RTIs. Multivariate analysis showed that women's age, education, occupation, dysmenorrhea, number of deliveries, personal hygiene habit, menstrual cycle, menstruation, abortion, the interval between abortion and sexual intercourse afterwards, RTI knowledge and the frequency of sexual intercourse per month were all related to RTIs in their sample [19].

Bacterial flora and mycosis of the vagina was studied by Turovskiy et al. 2011, to estimate the microbiological profile of vaginal flora and its association with any of the suspected demographic criteria in women complaining from pathological symptoms (pruritus, burning, vaginal discharge). They concluded that the common reason of vaginitis is bacterial infections. Both, place of living and women’s age influence the type of etiological factor [20]. However, our findings were not in consistency with this result as the p value of our result concerning residence versus infection was 0.387, which was statistically non-significant.

5. Conclusion

The most frequent infection among Sudanese women complain with vaginal discharge is bacterial vaginosis and Candida albicans. Pap smear had a relatively low predictive value for the presence of vaginal pathogens among women complaining of vaginal discharge.

Competing Interests

The authors have no competing interests with the work presented in this manuscript.

Ethics Approval

All patients were assured that all their obtained information will be handled in a confidential atmosphere and it will not affect their life after taking verbal and written consent. Ethical clearance and protocol approval were obtained from research and ethics committee of University of Medical Sciences, Sudan.

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in Brazil 2013.


  1. Faro S (1989) Vaginitis and pregnancy. Reprod Med 34: 602-604. View
  2. Brunham RC, Holmes KK, Embree E (1990) Sexually transmitted diseases in pregnancy. In: Holmes KK, March P-A, Sparling PF, Wiener PJ (eds): Sexually Transmitted Diseases. McGraw-Hill Information Services. pp 171- 801.
  3. Adad SJ, de Lima RV, Sawan ZT, Silva ML, de Souza MA, et al. (2001) Frequency of Trichomonas vaginalis, Candida sp and Gardnerella vaginalis in cervical-vaginal smears in four different decades. Sao Paulo Med J 119: 200-205. View
  4. Maria C, Zahra R, Sara P (2013) Prevalence of cervical-vaginal infections in the pap-smear samples in Iran. Glob J Health Sci 6: 201-206. View
  5. Summers PR, Sharp HT (1993) The management of obscure or difficult cases of vulvovaginitis. Clin Obstet Gynecol 36: 206-214. View
  6. Escoffery CT, Sinclair PA, Guthrie W (2001) Vaginitis emphysematosa associated with an abnormal Pap smear. West Indian Med J 50: 234-235. View
  7. Nokiani FA, Akbari H, Rezaei M, Madani H, Ale Agha ME (2008) Cost-- effectiveness of pap smear in Kermanshah, Iran. Asian Pac J Cancer Prev 9: 107-110. View
  8. Amsel R, Totten PA, Spiegel CA, Chen KCS, Eschenbach D, Holmes KK (1983) Nonspecific vaginitis: diagnostic criteria and microbial and epidemiologic associations. Am J Med 74: 14-22.
  9. Hillier SL, Krohn MA, Nugent RP, Gibbs RS (1992) Characteristics of three vaginal flora patterns assessed by gram stain among pregnant women. Vaginal Infections and Prematurity Study Group. Am J Obstet Gynecol 166: 938-944. View
  10. Akingbade OA, Akinjinmi AA, Awoderu OB, Okerentugba PO, Okonko IO (2013) Prevalence of Candida albicans Amongst Women Attending Health CentresIn Abeokuta, Ogun State, Nigeria. N Y Sci J 6: 53-59.
  11. Larsen B, Monif GR (2001) Understanding the bacterial flora of the female genital tract. Clin Infect Dis 32: e69-77. View
  12. Sadeghi M, Naeini KM (2001) Prevalence of Trichomonasis in women referred to health centers for pap-smear exam in Charmahal-Bakhtiari province. Shahrekord University of Medical Sciences Journal 3: 37-41.
  13. Namazi A, Sehatti F, Pour MA, Mazloumi AA (2005) Prevalence and risk factors for candida infection in women referred to health centers in Tabriz. Yazd University of Medical Sciences Journal 15: 61-67.
  14. Hernández-Martínez F, Hernández-García JA, Martínez-Peña MD, Muñíz- Becerril BL, Hernández-Cortez C, et al. (2013) Aetiology and frequency of cervico-vaginal infections among Mexican women African Journal of Microbiology Research 7: 27-34.
  15. Abdelaziz ZA, Ibrahim ME, Bilal NE, Hamid ME (2014) Vaginal infections among pregnant women at Omdurman Maternity Hospital in Khartoum. J Infect Dev Ctries 8: 490-497. View
  16. Gouya MM, Nabaei S (2005) Frequency of some sexually transmitted infections in a family planning clinic. Iran University of Medical Sciences Journal: 14.
  17. Jespers V, van de Wijgert J, Cools P, Verhelst R, Verstraelen H, et al. (2015) The significance of Lactobacillus crispatus and L. vaginalis for vaginal health and the negative effect of recent sex: a cross-sectional descriptive study across groups of African women. BMC Infect Dis 15: 115. View
  18. Rathod SD, Klausner JD, Krupp K, Reingold AL, Madhivanan P (2012) Epidemiologic Features of Vulvovaginal Candidiasis among Reproductive- Age Women in India. Infectious Diseases in Obstetrics and Gynecology 859071. View
  19. Zhang XJ, Shen Q, Wang GY, Yu YL, Sun YH, et al. (2009) Risk factors for reproductive tract infections among married women in rural areas of Anhui Province, China. Eur J Obstet Gynecol Reprod Biol 147: 187-191. View
  20. Turovskiy Y, Sutyak Noll K, Chikindas ML (2011) The aetiology of bacterial vaginosis. J Appl Microbiol 110: 1105-1128. View