Abstract

Hematopoietic stem cell transplantation is commonly used to treat several oncohematologic disease. The critical importance of cell dose in the clinical outcome, after transplant, has motivated the need to develop techniques aimed at reducing cell losses and increasing reproducibility.
Moreover, it has been clearly demonstrated that T-cells in a graft are directly correlated to the risk of developing a life-threatening GvHD in allotransplanted patients. Different techniques have been employed for this purpose, including physical, immunological and combined immunological/physical methods with different results in terms of purity, recovery, and time consumption.
To find new methods of cell manipulation is on of the most ambitious challenges in the field of stem cell transplantation.