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International Journal of Clinical & Medical Microbiology Volume 1 (2016), Article ID 1:IJCMM-108, 8 pages
https://doi.org/10.15344/2456-4028/2016/108
Review Article
Diversity of the Oral Microbiome and Dental Health and Disease

Bishoy Nasry1+, Carissa Choong1+, Erin Flamiatos1+, Jennifer Chai1+, Namgu Kim1+, Shay Strauss2, Tom Maier2,3 and Curtis A. Machida2,4*

1Academic DMD Program, Oregon Health & Science University (OHSU), 2730 Moody Avenue, Portland, OR 97201, USA
2Department of Integrative Biosciences, Oregon Health & Science University (OHSU), 2730 Moody Avenue, Portland, OR 97201, USA
3Department of Pathology and Radiology, Oregon Health & Science University (OHSU), 2730 Moody Avenue, Portland, OR 97201, USA
4Department of Pediatric Dentistry, Oregon Health & Science University (OHSU), 2730 Moody Avenue, Portland, OR 97201, USA
+Equal contributors to this work
Dr. Curt Machida, Department of Integrative Biosciences, OHSU School of Dentistry, 2730 SW Moody Avenue, Portland, OR 97201-5042, USA, Tel: 503-494-0034; E-mail: machidac@ohsu.edu
06 June 2016; 07 August 2016; 09 August 2016
Nasry B, Choong C, Flamiatos E, Chai J, Kim N, et al. (2016) Diversity of the Oral Microbiome and Dental Health and Disease. Int J Clin Med Microbiol 1: 108. doi: https://doi.org/10.15344/2456-4028/2016/108

References

  1. Lederberg J, McCray AT (2001) ‘Ome Sweet ‘Omics - a genealogical treasury of words. Scientist 15: 8 [Google Scholar]
  2. Turnbaugh PJ, Ley RE, Hamady M, Fraser-Liggett C, Knight R, et al. (2007) The human microbiome project: exploring the microbial part of ourselves in a changing world. Nature 449: 804-810 [CrossRef] [PubMed]
  3. The Human Microbiome Project Consortium (2012) Structure, Function and Diversity of the Healthy Human Microbiome. Nature 486: 207-214 [CrossRef] [Google Scholar] [PubMed]
  4. Zarco M, Vess T, Ginsburg G (2012) The oral microbiome in health and disease and the potential impact on personalized dental medicine. Oral Dis 18: 109-120 [CrossRef] [Google Scholar] [PubMed]
  5. Cho I, Blaser MJ (2012) The Human Microbiome: at the interface of health and disease. Nat Rev Genet 13: 260-270 [CrossRef] [Google Scholar] [PubMed]
  6. Wade W (2013) The oral microbiome in health and disease. Pharmacol Res 69: 137-143 [CrossRef] [Google Scholar] [PubMed]
  7. Dewhirst FE, Chen T, Izard J, Paster BJ, Tanner ACR (2010) The Human Oral Microbiome. J Bacteriol 192: 5002-5017 [CrossRef] [Google Scholar] [PubMed]
  8. Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE (2005) Defining the Normal Bacterial Flora of the Oral Cavity. J Clin Microbiol 43: 5721-5732 [CrossRef] [Google Scholar] [PubMed]
  9. Avila M, Ojcius DM, Yilmaz Ö (2009) The Oral Microbiota: Living with a Permanent Guest. DNA Cell Biol 28: 405-411 [CrossRef] [Google Scholar] [PubMed]
  10. Parahitiyawa NB, Scully C, Leung WK, Yam WC, Jin LJ, et al. (2010) Exploring the oral bacterial flora: current status and future directions. Oral Dis 16: 136-145 [CrossRef] [Google Scholar] [PubMed]
  11. Sutherland IW (2001) The biofilm matrix–an immobilized but dynamic microbial environment. Trends Microbiol 9: 222-227 [CrossRef] [Google Scholar] [PubMed]
  12. Xiao J, Klein MI, Falsetta ML, Lu B, Delahunty CM, et al. (2012) The Exopolysaccharide Matrix Modulates the Interaction between 3D Architecture and Virulence of a Mixed-Species Oral Biofilm. PLoS Pathog 8: e1002623 [CrossRef] [Google Scholar] [PubMed]
  13. Marsh PD (2004) Dental plaque as a microbial biofilm. Caries Res 38: 204- 211 [CrossRef] [Google Scholar] [PubMed]
  14. Costerton JW (2007) The biofilm primer. Springer Science & Business Media [Google Scholar]
  15. Larsen T (2002) Susceptibility of Porphyromonas gingivalis in biofilms to amoxicillin, doxycycline and metronidazole. Oral Microbiol Immunol 17: 267-271 [CrossRef] [Google Scholar] [PubMed]
  16. Stewart PS, Costerton JW (2001) Antibiotic resistance of bacteria in biofilms. Lancet 358: 135-138 [CrossRef] [Google Scholar] [PubMed]
  17. Høiby N, Bjarnsholt T, Givskov M, Molin S, Ciofu O (2010) Antibiotic resistance of bacterial biofilms. Int J of Antimicrob Agents 35: 322-332 [CrossRef] [Google Scholar] [PubMed]
  18. Mah TF, O’Toole GA (2001) Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol 9: 34-39 [CrossRef] [Google Scholar] [PubMed]
  19. Davies D (2003) Understanding biofilm resistance to antibacterial agents. Nat Rev Drug Discov 2: 114-122 [CrossRef] [Google Scholar] [PubMed]
  20. O’Connell HA, Kottkamp GS, Eppelbaum JL, Stubblefield BA, Gilbert SE, et al. (2006) Influences of Biofilm Structure and Antibiotic Resistance Mechanisms on Indirect Pathogenicity in a Model Polymicrobial Biofilm. Appl Environ Microbiol 72: 5013-5019 [CrossRef] [Google Scholar] [PubMed]
  21. Budhani RK, Struthers JK (1998) Interaction of Streptococcus pneumoniae and Moraxella catarrhalis: Investigation of the Indirect Pathogenic Role of β-Lactamase-Producing Moraxellae by Use of a Continuous-Culture Biofilm System. Antimicrob Agents Chemother 42: 2521-2526 [CrossRef] [Google Scholar] [PubMed]
  22. Armbruster CE, Hong W, Pang B, Weimer KE, Juneau RA, et al. (2010) Indirect Pathogenicity of Haemophilus Influenza and Moraxella catarrhalis in Polymicrobial Otitis Media Occurs via Interspecies Quorum Signaling. MBio 1: e00102-10 [CrossRef] [Google Scholar] [PubMed]
  23. Stoodley P, Sauer K, Davies SG, Costerton JW (2002) Biofilms as complex differentiated communities. Annu Rev Microbiol 56: 187-209 [CrossRef] [Google Scholar] [PubMed]
  24. Eaton KA, Ower P (2015) Practical Periodontics. Elsevier Health Sciences UK
  25. Flemming HC, Wingender J (2010) The biofilm matrix. Nat Rev Microbiol 8: 623-633 [CrossRef] [Google Scholar] [PubMed]
  26. Marsh PD, Devine DA (2011) How is the development of dental biofilms influenced by the host? J Clin Periodontol 38: 28-35 [CrossRef] [Google Scholar] [PubMed]
  27. Jenkinson HF, Douglas LJ (2002) Interactions between Candida Species and Bacteria in Mixed Infections. Polymicrobial Diseases Chapter 18. Washington (DC): ASM Press [Google Scholar]
  28. Kennedy MJ (1981) Inhibition of Candida albicans by the anaerobic oral flora of mice in vitro. Sabouraudia 19: 205-208 [CrossRef] [Google Scholar] [PubMed]
  29. Wilks M (2007) Bacteria and early human development. Early Hum Dev 83:165-170 [CrossRef] [Google Scholar] [PubMed]
  30. Darby ML, Walsh M (2014) Dental hygiene: theory and practice. Elsevier Health Sciences
  31. Takahashi N, Nyvad B (2011) The role of bacteria in the caries process: ecological perspectives. J Dent Res 90: 294-303 [CrossRef] [Google Scholar] [PubMed]
  32. Selwitz RH, Ismali AI, Pitts NB (2007) Dental caries. Lancet 369: 51-59 [CrossRef] [Google Scholar] [PubMed]
  33. Peterson PE, Bourgeois D, Ogawa H, Estupinan-Day S, Ndiaye C (2005) The global burden of oral diseases and risks to oral health. Bull World Health Organ 83: 661-669 [CrossRef] [Google Scholar] [PubMed]
  34. US Department of Health and Human Services (2000) Oral Health in America: A Report of the Surgeon General. Rockville, MD: US Department of Health and Human Services, National Institutes of Health, National Institute of Dental and Craniofacial Research
  35. Dental Caries (Tooth Decay) in Adults (Age 20 to 64)
  36. Paes Leme AF, Koo H, Bellato CM, Bedi G, Cury JA (2006) The Role of Sucrose in Cariogenic Dental Biofilm Formation – New Insight. J Dent Res 85: 878-887 [CrossRef] [Google Scholar] [PubMed]
  37. Featherstone JD (2004) The continuum of dental caries evidence for a dynamic disease process. J Dent Res 83: C39-C42 [CrossRef] [Google Scholar] [PubMed]
  38. Marsh PD (1994) Microbial ecology of dental plaque and its significance in health and disease. Adv Dent Res 8: 263-271 [CrossRef] [Google Scholar] [PubMed]
  39. Marsh PD (2006) Dental plaque as a biofilm and a microbial community – implications for health and disease. BMC Oral Health 6: S14 [CrossRef] [Google Scholar] [PubMed]
  40. Aas JA, Griffen AL, Dardis SR, Lee AM, Olsen I, et al. (2008) Bacteria of Dental Caries in Primary and Permanent Teeth in Children and Young Adults. J Clin Micro Biol 46: 1407-1417 [CrossRef] [Google Scholar] [PubMed]
  41. Jenkinson HF (2011) Beyond the oral microbiome. Environ Microbiol 13: 3077-3087 [CrossRef] [Google Scholar] [PubMed]
  42. ten Cate JM, Duijsters PPE (1982) Alternating Demineralization and Remineralization of Artificial Enamel Lesions. Caries Res 16: 201-210 [CrossRef] [Google Scholar] [PubMed]
  43. Featherstone JDB (2008) Dental caries: a dynamic disease process. Aust Dent J 53: 286-291 [CrossRef] [Google Scholar] [PubMed]
  44. García-Godoy F, Hicks MJ (2008) Maintaining the integrity of the enamel surface: the role of dental biofilm, saliva and preventive agents in enamel demineralization and remineralization. J Am Dent Assoc 139: 25S-34S [CrossRef] [Google Scholar] [PubMed]
  45. Featherstone JD (1999) Prevention and reversal of dental caries: role of low level fluoride. Community Dent Oral Epidemiol 27: 31-40 [CrossRef] [Google Scholar] [PubMed]
  46. Rôças IN, Alves FR, Rachid CT, Lima KC, Assunção IV, et al. (2016) Microbiome of Deep Dentinal Caries Lesions in Teeth with Symptomatic Irreversible Pulpitis. PLoS One 11: e0154653 [CrossRef] [Google Scholar] [PubMed]
  47. Jagathrakshakan SN, Sethumadhava RJ, Mehta DT, Ramanathan A (2015) 16S rRNA gene-based metagenomic analysis identifies a novel bacterial co-prevalence pattern in dental caries. Eur J Dent 9: 127-132 [CrossRef] [Google Scholar] [PubMed]
  48. Lee HS, Lee JH, Kim SO, Song JS, Kim BI, et al. (2016) Comparison of the Oral Microbiome of Siblings Using Next-generation Sequencing: a Pilot Study. Oral Dis 22: 549-556 [CrossRef] [Google Scholar] [PubMed]
  49. Kianoush N, Adler CJ, Nguyen KAT, Browne GV, Simonian M, et al. (2014) Bacterial profile of dentine caries and the impact of pH on bacterial population diversity. PLoS One 9: e92940 [CrossRef] [Google Scholar] [PubMed]
  50. Gross EL, Leys EJ, Gasparovich SR, Firestone ND, Schwartzbaum JA, et al. (2010) Bacterial 16S Sequence Analysis of Severe Caries in Young Permanent Teeth. J Clin Microbiol 48: 4121-4128 [CrossRef] [Google Scholar] [PubMed]
  51. Beighton D, Al-Haboubi M, Mantzourani M, Gilbert SC, Clark D, et al. (2010) Oral Bifidobacteria: caries-associated bacteria in older adults. J Dent Res 89: 970-974 [CrossRef] [Google Scholar] [PubMed]
  52. Banas JA (2004) Virulence properties of Streptococcus mutans. Front Biosci 9: 1267-1277 [CrossRef] [Google Scholar] [PubMed]
  53. Kreth J, Zhang Y, Herzberg MC (2008) Streptococcal antagonism in oral biofilms: Streptococcus sanguinis and Streptococcus gordonii interference with Streptococcus mutans. J Bacteriol 190: 4632-4640 [CrossRef] [Google Scholar] [PubMed]
  54. Huang R, Li M, Gregory RL (2011) Bacterial interactions in dental biofilm. Virulence 2: 435-444 [CrossRef] [Google Scholar] [PubMed]
  55. Hirose H, Hirose K, Isogai E, Miura H, Ueda I (1993) Close association between Streptococcus sobrinus in the saliva of young children and smooth-surface caries increment. Caries Res 27: 292-297 [CrossRef] [Google Scholar] [PubMed]
  56. Conrads G, de Soet JJ, Song L, Henne K, Sztajer H, et al. (2014) Comparing the cariogenic species Streptococcus sobrinus and S. mutans on whole genome level. J Oral Microbiol 6 [CrossRef] [Google Scholar] [PubMed]
  57. deSoet JJ, van Loveren C, Lammens AJ, Pavicić MJ, Homburg CH, et al. (1991) Differences in cariogenicity between fresh isolates of Streptococcus sobrinus and Streptococcus mutans. Caries Res 25: 116-122 [CrossRef] [Google Scholar] [PubMed]
  58. Johansson I, Witkowska E, Kaveh B, Holgerson PL, Tanner ACR (2016) The Microbiome in Populations with a Low and High Prevalence of Caries. J Dent Res 95: 80-86 [CrossRef] [Google Scholar] [PubMed]
  59. Almståhl A, Lingström P, Eliasson L, Carlén A (2013) Fermentation of sugars and sugar alcohols by plaque Lactobacillus strains. Clin Oral Investig 17: 1465-1470 [CrossRef] [Google Scholar] [PubMed]
  60. Kralj S, van Geel-Schutten GH, Dondorff MM, Kirsanovs S, van der Maarel MJ, et al. (2004) Glucan synthesis in the genus Lactobacillus: isolation and characterization of glucan sucrase genes, enzymes and glucan products from six different strains. Microbiol 150: 3681-3690 [CrossRef] [Google Scholar] [PubMed]
  61. Badet C, Thebaud NB (2008) Ecology of lactobacilli in the oral cavity: a review of literature. Open Microbiol J 2: 38 [CrossRef] [Google Scholar] [PubMed]
  62. Caufield PW, Schön CN, Saraithong P, Li Y, Argimón S (2015) Oral Lactobacilli and Dental Caries: A Model for Niche Adaptation in Humans. J Dent Res 94: 110S-118S [CrossRef] [Google Scholar] [PubMed]
  63. Costalonga M, Herzberg MC (2014) The oral microbiome and the immunobiology of periodontal disease and caries. Immun Let 162: 22-38 [CrossRef] [Google Scholar] [PubMed]
  64. Hoshino E (1985) Predominant obligate anaerobes in human carious dentin. J Dent Res 64: 1195-1198 [CrossRef] [Google Scholar] [PubMed]
  65. Becker MR, Paster BJ, Leys EJ, Moeschberger ML, Kenyon SG, et al. (2002) Molecular analysis of bacterial species associated with childhood caries. J Clin Microbiol 40: 1001-1009 [CrossRef] [Google Scholar] [PubMed]
  66. Mantzourani M, Gilbert SC, Sulong HN, Sheehy EC, Tank S, et al. (2009) The isolation of bifidobacteria from occlusal carious lesions in children and adults. Caries Res 43: 308-313 [CrossRef] [Google Scholar] [PubMed]
  67. Henne K, Rheinberg A, Melzer-Krick B, Conrads G (2015) Aciduric microbial taxa including Scardovia wiggsiae and Bifidobacterium spp. in caries and caries free subjects. Anaerobe 35: 60-65 [CrossRef] [Google Scholar] [PubMed]
  68. Tanner AC (2015) Anaerobic culture to detect periodontal and caries pathogens. J Oral Biosci 57: 18-26 [CrossRef] [Google Scholar] [PubMed]
  69. Tanner AC, Mathney JMJ, Kent RL Jr, Chalmers NI, Hughes CV, et al. (2011) Cultivable anaerobic microbiota of severe early childhood caries. J Clin Microbiol 49: 1464-1474 [CrossRef] [Google Scholar] [PubMed]
  70. Vacharaksa A, Suvansopee P, Opaswanich N, Sukarawan W (2015) PCR detection of Scardovia wiggsiae in combination with Streptococcus mutans for early childhood caries-risk prediction. Eur J Oral Sci123: 312-318 [CrossRef] [Google Scholar] [PubMed]
  71. Tanner AC, Kent RL, Holgerson PL, Hughes CV, Loo CY, et al. (2011) Microbiota of severe early childhood caries before and after therapy. J Dent Res 90: 1298-1305 [CrossRef] [Google Scholar] [PubMed]
  72. Nadkarni MA, Caldon CE, Chhour KL, Fisher IP, Martin FE et al. (2004) Carious dentine provides a habitat for a complex array of novel Prevotellalike bacteria. J Clin Microbiol 42: 5238-5244 [CrossRef] [Google Scholar] [PubMed]
  73. Siqueira JF, Rôcas IN (2011) Microbiology and treatment of endodontic infections. In: Hargreaves K, Berman L, eds. Cohen’s pathway to the pulp, tenth ed. St. Louis MO: Mosby Inc.; p. 559-604
  74. Endodontic Diagnosis. Endodontics: Colleagues for Excellence Newsletter 2013: American Association of Endodontists. Web. 15 May 2016
  75. Gomes BP, Berber VB, Kokaras AS, Chen T, Paster BJ (2015) Microbiomes of Endodontic-Periodontal Lesions before and after Chemomechanical Preparation. J Endod 41: 1975-1984 [CrossRef] [Google Scholar] [PubMed]
  76. Rôcas IN, Lima KC, Assuncao IV, Gomes PN, Bracks IV, et al. (2015) Advanced caries microbiota in teeth with Irreversible pulpitis. J Endod 41: 1450-1455 [CrossRef] [Google Scholar] [PubMed]
  77. Martin FE, Nadkarni MA, Jacques NA, Hunter N (2002) Quantitative Microbiological Study of Human Carious Dentine by Culture and Real-Time PCR: Association of Anaerobes with Histopathological Changes in Chronic Pulpitis. J Clin Microbiol 40: 1698-1704 [CrossRef] [Google Scholar] [PubMed]
  78. George N, Flamiatos E, Kawasaki K, Kim N, Carriere C, et al. (2016) Oral Microbiota Species in Acute Apical Endodontic Abscesses. J Oral Microbiol 8: 30989 [CrossRef] [Google Scholar] [PubMed]
  79. Sakamoto M, Siqueira JF, Rôcas IN, Benno Y (2009) Diversity of Spirochetes in Endodontic Infections. J Clin Microbiol 47: 1352-1357 [CrossRef] [Google Scholar] [PubMed]
  80. Tennert C, Fuhrmann M, Wittmer A, Karygianni L, Altenburger MJ, et al. (2014) New bacterial composition in primary persistent/secondary endodontic infections with respect to clinical and radiographic findings. J Endod 40: 670-677 [CrossRef] [Google Scholar] [PubMed]
  81. George M, Ivančaková R (2007) Root Canal Microflora. Acta Medica (Hradec Kralove) 50: 7-15 [Google Scholar] [PubMed]
  82. Leite FR, Nascimento GG, Demarco FF, Gomes BP, Pucci CR, et al. (2015) Prevalence of Treponema Species Detected in Endodontic Infections: Systematic Review and Meta-regression Analysis. J Endod 41: 579-587 [CrossRef] [Google Scholar] [PubMed]
  83. Kovac J, Kovac D, Slobodnikova L, Kotulova D (2013) Enterococcus faecalis and Candida albicans in the dental root canal and periapical infections. Bratisl Lek Listy 114: 716-720 [Google Scholar] [PubMed]
  84. Rôcas IN, Siqueira JF, Santos KR (2004) Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod 30: 315-320 [CrossRef] [Google Scholar] [PubMed]
  85. Sakamoto M, Rocas IN, Siqueira JF, Benno Y (2006) Molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. Oral Microbiol Immunol 21: 11-122 [CrossRef] [Google Scholar] [PubMed]
  86. Loesche W (2007) Dental caries and periodontitis: contrasting two infections that have medical implications. Infect Dis Clin North 21: 471-502 [CrossRef] [Google Scholar] [PubMed]
  87. Loesche W, Grossman NS (2001) Periodontal disease as a specific, albeit chronic, infection: diagnosis and treatment. Clin Microbiol Rev 14: 727-752 [CrossRef] [Google Scholar] [PubMed]
  88. Slots J (1979) Subgingival microflora and periodontal disease. J Clin Periodontol 6: 351-382 [CrossRef] [Google Scholar] [PubMed]
  89. Löe H, Theilade E, Jensen SB (1965) Experimental gingivitis in man. J Periodontol 36: 177-187 [CrossRef] [Google Scholar] [PubMed]
  90. Armitage GC (1999) Development of a classification system for periodontal diseases and conditions. Ann Periodontol 4: 1-6 [CrossRef] [Google Scholar] [PubMed]
  91. American Academy of Periodontology Task Force Report on the Update to the 1999 Classification of Periodontal Diseases and Conditions (2015) J Periodontol 86: 835-838 [CrossRef] [Google Scholar] [PubMed]
  92. Holt SC, Ebersole JL (2005) Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia: the ‘red complex’, a prototype polybacterial pathogenic consortium in periodontitis. Periodontol 38: 72- 122 [CrossRef] [Google Scholar] [PubMed]
  93. Genco RJ (1992) Host Responses in Periodontal Diseases: Current Concepts. J Periodontol 63: 338-355 [CrossRef] [Google Scholar] [PubMed]
  94. Genco RJ (1996) Current View of Risk Factors for Periodontal Diseases. J Periodontol 67: 1041-1049 [CrossRef] [Google Scholar] [PubMed]
  95. Lamont R, Jenkinson H (1998) Life Below the Gum Line: Pathogenic Mechanisms of Porphyromonas gingivalis. Microbiol Mol Biol Rev 62: 1244-1263 [Google Scholar] [PubMed]
  96. Zhu W, Lee S (2016) Surface interactions between two of the main periodontal pathogens: Porphyromonas gingivalis and Tannerella forsythia. J Periodontal Implant Sci 46: 2-9 [CrossRef] [Google Scholar] [PubMed]
  97. Nilius A, Spencer S, Simonson L (1993) Stimulation of in vitro growth of Treponema denticola by extracellular growth factors produced by Porphyromonas gingivalis. J Dent Res 72: 1027-1031 [CrossRef] [Google Scholar] [PubMed]
  98. Sela MN (2001) Role of Treponema Denticola in Periodontal Diseases. Crit Rev Oral Biol Med 12: 399-413 [CrossRef] [Google Scholar] [PubMed]
  99. Boehringer H, Taichman N, Shenker B (1984) Suppression of Fibroblast Proliferation by Oral Spirochetes. Infect Immun 45: 155-159 [Google Scholar] [PubMed]
  100. Uitto V, Grenier D, Chan E, McBride B (1988) Isolation of chymotrypsin-like enzyme from Treponema denticola. Infect Immun 56: 2717-2722 [Google Scholar] [PubMed]
  101. Rosen G, Sela M, Naor R, Halabi A, Barak V, et al. (1999) Activation of murine macrophages by lipoprotein and lipooligosaccharide of Treponema denticola. Infect Immun 67: 1180-1186 [Google Scholar] [PubMed]
  102. Kawamoto D, Ando-Suguimoto ES, Bueno-Silva B, DiRienzo JM, Mayer MP (2016) Alteration of Homeostasis in Pre-osteoclasts Induced by Aggregatibacter actinomycetemcomitans CDT. Front Cell Infect Microbiol 6: 33 [CrossRef] [Google Scholar] [PubMed]
  103. Fine D, Markowitz K, Fairlie K, Tischio-Bereski D, Ferrendiz J, et al. (2013) A Consortium of Aggregatibacter actinomycetemcomitans, Streptococcus parasanguinis, and Filifactor alocis Is Present in Sites Prior to Bone Loss in a Longitudinal Study of Localized Aggressive Periodontitis. J Clin Microbiol 51: 2850-2861 [CrossRef] [Google Scholar] [PubMed]
  104. FineD, Markowitz K, Furgang D, Fairlie K, Ferrandiz J, et al. (2007) Aggregatibacter actinomycetemcomitans and Its Relationship to Initiation of Localized Aggressive Periodontitis: Longitudinal Cohort Study of Initially Healthy Adolescents. J Clin Microbiol 45: 3859-3869 [CrossRef] [Google Scholar] [PubMed]
  105. Aruni AW, Roy F, Fletcher HM (2011) Filifactor alocis Has Virulence Attributes That Can Enhance Its Persistence under Oxidative Stress Conditions and Mediate Invasion of Epithelial Cells by Porphyromonas gingivalis. Infect Immun 79: 3872-3886 [CrossRef] [Google Scholar] [PubMed]
  106. Castillo A, Mira A, Pico A, Nibali L, Henderson B, et al. (2015) Subgingival microbiota in health compared to periodontitis and the influence of smoking. Front Microbiol 6: 119 [CrossRef] [Google Scholar] [PubMed]