Figure 1: Histone H1 detection kit (Q-may Laboratory Corporation, Oita, Japan). In an acute rejection model of orthotopic rat liver transplantation (OLT; DA donor into LEW recipient) model, severe acute rejection was obseved on post-transplant day 7 in LEW (RT1l) rats bearing DA (RT1a) livers. 100 microlitter of serm from naive LEW rats or OLT (DA-LEW) was applied to the kit. Histone H1 was not detected in serum of LEW rats while it was clearly detected in serum taken on post-operative day (POD) 7 after OLT(DA-LEW).