Table 2: Characterization of phenolic compounds of the spent hops extract using their spectral characteristic in UPLC-DAD (retention time, λmax) and negative ions in UPLC–ESI-MS.
Compounds tR (min) λmax (nm) [M-H]- [MS-MS-] (m/z)
Neochlorogenic acid 2.30 320 353 235/191/147
B-type PA-trimer 2.57 280 865 577/407/289
B3 dimer 2.64 280 577 407/289
B-type PA-trimer 2.98 280 865 577/407/289
(+)- Catechin 3.11 280 289 245/203
B1 dimer 3.38 280 577 407/289
Chlorogenic acid 3.50 320 353 235/191/147
B2-dimer 3.87 280 577 407/289
Cryptochlorogenic acid 3.50 320 353 235/191/147
Feruloylquinic acid 3.98 322 367
(-)-Epicatechin 4.65 280 289 245/203
Procyanidin C1 5.04 280 865 577/407/289
B4 dimer 5.27 280 577 407/289
B-type PA-trimer 5.42 280 865 577/407/289
Quercetin-3-hexosylrutinoside 5.59 353 771 609/591/301
Quercetin-3-dihexoside 6.00 353 625 463/301
Quercetin-hexosiderhamnoside-rhamnoside 6.23 353 755 609/463/301
Quercetin-hexosiderhamnoside 6.60 355 609 463/301
Quercetin-hexoside 7.10 355 463 301
1-[2(Methylpropanoyl) phloroglucinyl] glucoside (multifidol-glucoside) 7.14 287 358 196
Quercetin-hexosiderhamnoside 7.25 355 609 463/301
Quercetin-3-O-hexoside 7.36 355 463 301
Quercetin-O-deoxyhexose-Odeoxyhexoside 7.50 355 593 447/301
Quercetin-3-O-acetylhexoside 8.02 350 506 463/445/301
Kempferol-3-O-rutinoside 8.35 340 593 447/327/285
Kempferol-3-O-hexoside 8.52 340 447 327/285
Kaempferol -3-O-acetylhexoside 9.37 340 489 285