Figure 5: In vitro evaluation of antimicrobial activity by biofilm formation assay. Fluorescence in micrograph (LIVE/DEAD staining) of CPMs (0) and CPMs (1000) disc surface after in vitro biofilm formation. E. coli (a) and S. aureus (b) were cultured on CPMs (0) or CPMs (1000) discs. Vital bacteria appear green (SYTO9) and dead bacteria appear red (PI). Right panels represent merged image of SYTO9 and PI. Bar indicates 20 μm.