Figure 2: Impact of HSA delipidation on biochemical properties.
a) unmodified primary amino group level in HSA determined by trinitrobenzene sulphonic acid (TNBS) assay;
b) carbonyl level was assessed by spectrophotometric assay;
c) free thiol group level of HSA assessed by Ellman’s method; Values are means ± S.D. for three experiments. Significance of differences compared with fatty acid purified albumin (vs. FA-HSAP): ***P < 0.001.
a) unmodified primary amino group level in HSA determined by trinitrobenzene sulphonic acid (TNBS) assay;
b) carbonyl level was assessed by spectrophotometric assay;
c) free thiol group level of HSA assessed by Ellman’s method; Values are means ± S.D. for three experiments. Significance of differences compared with fatty acid purified albumin (vs. FA-HSAP): ***P < 0.001.
