Figure 1: Biochemical and structural characterization of human albumin purified by different methods (chromatography: Col Chrom; batch: Batch Chrom and AS precipitation: AS).
a) coomassie staining of purified HSA fractions obtained following three methods and separated by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE) (12%). The arrow indicates the localization of the dimeric form of HSA. ;
b) unmodified primary amino group level in HSA determined by trinitrobenzene sulphonic acid (TNBS) assay;
c) free thiol group level of HSA assessed by Ellman’s method;
d) Fluorescence spectra of tryptophan with excitation wavelength at 270 nm.
a) coomassie staining of purified HSA fractions obtained following three methods and separated by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE) (12%). The arrow indicates the localization of the dimeric form of HSA. ;
b) unmodified primary amino group level in HSA determined by trinitrobenzene sulphonic acid (TNBS) assay;
c) free thiol group level of HSA assessed by Ellman’s method;
d) Fluorescence spectra of tryptophan with excitation wavelength at 270 nm.
