A) The cellular content and the localization of cyclin D1 in cells proliferating for 24 h assessed by confocal microscopy. The images are representative of three separate experiments. Bar, 20μm. The integrated optical density (IOD) obtained in control (Ctrl) cells was set as 100%, and a nucleus/cytoplasm (N/C) ratio was calculated. The data are presented as means ± SE, with n=10/treatment conditions.
B) The levels of CycD1 in myoblasts proliferating for 24 h evaluated by immunoblotting (upper panel) and the levels of CycD1 bound to cyclin-dependent kinase 4 (Cdk4) assessed by immunoprecipitation (lower panel). The control probing with the antibody used for immunoprecipitation (anti-Cdk4) was also performed and the actin level as a loading control was presented. The means ± SE, with n=9/treatment conditions, and representative blots are shown. * - significantly different vs control value.
