Figure 4: PCR-denaturing gradient gel electrophoresis (DGGE) analysis of cecal microbiota, based on 16S rRNA gene sequences in the FF group(rats fed the fiber-free diet (FF diet)), PHGG group, (rats fed the FF diet with 5% PHGG (partially hydrolyzed guar gum)) andPHGG-FF group (rats fed the FF diet for 10 days after being fed the PHGG diet for 20 days).(A) DGGE gel image stained with ethidium bromide. (B) DGGE banding patterns were analyzed using FPQuest software (Bio-Rad). Similarity was visualised by dendograms using the unweighted pair group methodand arithmetic averages (UPGMA).